Indirect monitoring of TORC1 signalling pathway reveals molecular diversity among different yeast strains.
Identifieur interne : 000321 ( Main/Exploration ); précédent : 000320; suivant : 000322Indirect monitoring of TORC1 signalling pathway reveals molecular diversity among different yeast strains.
Auteurs : Eduardo I. Kessi-Pérez [Chili] ; Francisco Salinas [Chili] ; Jennifer Molinet [Chili] ; Asier González [Suisse] ; Sara Mu Iz [Espagne] ; José M. Guillam N [Espagne] ; Michael N. Hall [Suisse] ; Luis F. Larrondo [Chili] ; Claudio Martínez [Chili]Source :
- Yeast (Chichester, England) [ 1097-0061 ] ; 2019.
Descripteurs français
- KwdFr :
- Complexe-1 cible mécanistique de la rapamycine (génétique), Complexe-1 cible mécanistique de la rapamycine (métabolisme), Fermentation (MeSH), Gènes rapporteurs (MeSH), Luciferases (génétique), Phosphorylation (MeSH), Protéines de Saccharomyces cerevisiae (génétique), Protéines ribosomiques (génétique), Régions promotrices (génétique) (MeSH), Régulation de l'expression des gènes fongiques (MeSH), Saccharomyces cerevisiae (génétique), Saccharomyces cerevisiae (métabolisme), Transduction du signal (MeSH), Variation génétique (MeSH).
- MESH :
- génétique : Complexe-1 cible mécanistique de la rapamycine, Luciferases, Protéines de Saccharomyces cerevisiae, Protéines ribosomiques, Saccharomyces cerevisiae.
- métabolisme : Complexe-1 cible mécanistique de la rapamycine, Saccharomyces cerevisiae.
- Fermentation, Gènes rapporteurs, Phosphorylation, Régions promotrices (génétique), Régulation de l'expression des gènes fongiques, Transduction du signal, Variation génétique.
English descriptors
- KwdEn :
- Fermentation (MeSH), Gene Expression Regulation, Fungal (MeSH), Genes, Reporter (MeSH), Genetic Variation (MeSH), Luciferases (genetics), Mechanistic Target of Rapamycin Complex 1 (genetics), Mechanistic Target of Rapamycin Complex 1 (metabolism), Phosphorylation (MeSH), Promoter Regions, Genetic (MeSH), Ribosomal Proteins (genetics), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (metabolism), Saccharomyces cerevisiae Proteins (genetics), Signal Transduction (MeSH).
- MESH :
- chemical , genetics : Luciferases, Mechanistic Target of Rapamycin Complex 1, Ribosomal Proteins, Saccharomyces cerevisiae Proteins.
- chemical , metabolism : Mechanistic Target of Rapamycin Complex 1.
- genetics : Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- Fermentation, Gene Expression Regulation, Fungal, Genes, Reporter, Genetic Variation, Phosphorylation, Promoter Regions, Genetic, Signal Transduction.
Abstract
Saccharomyces cerevisiae is the main species responsible for the alcoholic fermentation in wine production. One of the main problems in this process is the deficiency of nitrogen sources in the grape must, which can lead to stuck or sluggish fermentations. Currently, yeast nitrogen consumption and metabolism are under active inquiry, with emphasis on the study of the TORC1 signalling pathway, given its central role responding to nitrogen availability and influencing growth and cell metabolism. However, the mechanism by which different nitrogen sources activates TORC1 is not completely understood. Existing methods to evaluate TORC1 activation by nitrogen sources are time-consuming, making difficult the analyses of large numbers of strains. In this work, a new indirect method for monitoring TORC1 pathway was developed on the basis of the luciferase reporter gene controlled by the promoter region of RPL26A gene, a gene known to be expressed upon TORC1 activation. The method was tested in strains representative of the clean lineages described so far in S. cerevisiae. The activation of the TORC1 pathway by a proline-to-glutamine upshift was indirectly evaluated using our system and the traditional direct methods based on immunoblot (Sch9 and Rps6 phosphorylation). Regardless of the different molecular readouts obtained with both methodologies, the general results showed a wide phenotypic variation between the representative strains analysed. Altogether, this easy-to-use assay opens the possibility to study the molecular basis for the differential TORC1 pathway activation, allowing to interrogate a larger number of strains in the context of nitrogen metabolism phenotypic differences.
DOI: 10.1002/yea.3351
PubMed: 30094872
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Guillam N</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Biotecnología de los Alimentos, Instituto de Agroquímica y Tecnología de los Alimentos (CSIC), Paterna, Valencia, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Departamento de Biotecnología de los Alimentos, Instituto de Agroquímica y Tecnología de los Alimentos (CSIC), Paterna, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Hall, Michael N" sort="Hall, Michael N" uniqKey="Hall M" first="Michael N" last="Hall">Michael N. 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Larrondo</name><affiliation wicri:level="1"><nlm:affiliation>Millennium Institute for Integrative Biology (iBio), Santiago, Chile.</nlm:affiliation><country xml:lang="fr">Chili</country><wicri:regionArea>Millennium Institute for Integrative Biology (iBio), Santiago</wicri:regionArea><wicri:noRegion>Santiago</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.</nlm:affiliation><country xml:lang="fr">Chili</country><wicri:regionArea>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago</wicri:regionArea><wicri:noRegion>Santiago</wicri:noRegion></affiliation></author><author><name sortKey="Martinez, Claudio" sort="Martinez, Claudio" uniqKey="Martinez C" first="Claudio" last="Martínez">Claudio Martínez</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Ciencia y Tecnología de los Alimentos, Universidad de Santiago de Chile (USACH), Santiago, Chile.</nlm:affiliation><country xml:lang="fr">Chili</country><wicri:regionArea>Departamento de Ciencia y Tecnología de los Alimentos, Universidad de Santiago de Chile (USACH), Santiago</wicri:regionArea><wicri:noRegion>Santiago</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Centro de Estudios en Ciencia y Tecnología de Alimentos (CECTA), Universidad de Santiago de Chile (USACH), Santiago, Chile.</nlm:affiliation><country xml:lang="fr">Chili</country><wicri:regionArea>Centro de Estudios en Ciencia y Tecnología de Alimentos (CECTA), Universidad de Santiago de Chile (USACH), Santiago</wicri:regionArea><wicri:noRegion>Santiago</wicri:noRegion></affiliation></author></analytic><series><title level="j">Yeast (Chichester, England)</title><idno type="eISSN">1097-0061</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fermentation (MeSH)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Genetic Variation (MeSH)</term><term>Luciferases (genetics)</term><term>Mechanistic Target of Rapamycin Complex 1 (genetics)</term><term>Mechanistic Target of Rapamycin Complex 1 (metabolism)</term><term>Phosphorylation (MeSH)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Ribosomal Proteins (genetics)</term><term>Saccharomyces cerevisiae (genetics)</term><term>Saccharomyces cerevisiae (metabolism)</term><term>Saccharomyces cerevisiae Proteins (genetics)</term><term>Signal Transduction (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Complexe-1 cible mécanistique de la rapamycine (génétique)</term><term>Complexe-1 cible mécanistique de la rapamycine (métabolisme)</term><term>Fermentation (MeSH)</term><term>Gènes rapporteurs (MeSH)</term><term>Luciferases (génétique)</term><term>Phosphorylation (MeSH)</term><term>Protéines de Saccharomyces cerevisiae (génétique)</term><term>Protéines ribosomiques (génétique)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Saccharomyces cerevisiae (génétique)</term><term>Saccharomyces cerevisiae (métabolisme)</term><term>Transduction du signal (MeSH)</term><term>Variation génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Luciferases</term><term>Mechanistic Target of Rapamycin Complex 1</term><term>Ribosomal Proteins</term><term>Saccharomyces cerevisiae Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mechanistic Target of Rapamycin Complex 1</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Complexe-1 cible mécanistique de la rapamycine</term><term>Luciferases</term><term>Protéines de Saccharomyces cerevisiae</term><term>Protéines ribosomiques</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Complexe-1 cible mécanistique de la rapamycine</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Fermentation</term><term>Gene Expression Regulation, Fungal</term><term>Genes, Reporter</term><term>Genetic Variation</term><term>Phosphorylation</term><term>Promoter Regions, Genetic</term><term>Signal Transduction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Fermentation</term><term>Gènes rapporteurs</term><term>Phosphorylation</term><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes fongiques</term><term>Transduction du signal</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Saccharomyces cerevisiae is the main species responsible for the alcoholic fermentation in wine production. One of the main problems in this process is the deficiency of nitrogen sources in the grape must, which can lead to stuck or sluggish fermentations. Currently, yeast nitrogen consumption and metabolism are under active inquiry, with emphasis on the study of the TORC1 signalling pathway, given its central role responding to nitrogen availability and influencing growth and cell metabolism. However, the mechanism by which different nitrogen sources activates TORC1 is not completely understood. Existing methods to evaluate TORC1 activation by nitrogen sources are time-consuming, making difficult the analyses of large numbers of strains. In this work, a new indirect method for monitoring TORC1 pathway was developed on the basis of the luciferase reporter gene controlled by the promoter region of RPL26A gene, a gene known to be expressed upon TORC1 activation. The method was tested in strains representative of the clean lineages described so far in S. cerevisiae. The activation of the TORC1 pathway by a proline-to-glutamine upshift was indirectly evaluated using our system and the traditional direct methods based on immunoblot (Sch9 and Rps6 phosphorylation). Regardless of the different molecular readouts obtained with both methodologies, the general results showed a wide phenotypic variation between the representative strains analysed. Altogether, this easy-to-use assay opens the possibility to study the molecular basis for the differential TORC1 pathway activation, allowing to interrogate a larger number of strains in the context of nitrogen metabolism phenotypic differences.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30094872</PMID><DateCompleted><Year>2019</Year><Month>04</Month><Day>19</Day></DateCompleted><DateRevised><Year>2019</Year><Month>04</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1097-0061</ISSN><JournalIssue CitedMedium="Internet"><Volume>36</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>01</Month></PubDate></JournalIssue><Title>Yeast (Chichester, England)</Title><ISOAbbreviation>Yeast</ISOAbbreviation></Journal><ArticleTitle>Indirect monitoring of TORC1 signalling pathway reveals molecular diversity among different yeast strains.</ArticleTitle><Pagination><MedlinePgn>65-74</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/yea.3351</ELocationID><Abstract><AbstractText>Saccharomyces cerevisiae is the main species responsible for the alcoholic fermentation in wine production. One of the main problems in this process is the deficiency of nitrogen sources in the grape must, which can lead to stuck or sluggish fermentations. Currently, yeast nitrogen consumption and metabolism are under active inquiry, with emphasis on the study of the TORC1 signalling pathway, given its central role responding to nitrogen availability and influencing growth and cell metabolism. However, the mechanism by which different nitrogen sources activates TORC1 is not completely understood. Existing methods to evaluate TORC1 activation by nitrogen sources are time-consuming, making difficult the analyses of large numbers of strains. In this work, a new indirect method for monitoring TORC1 pathway was developed on the basis of the luciferase reporter gene controlled by the promoter region of RPL26A gene, a gene known to be expressed upon TORC1 activation. The method was tested in strains representative of the clean lineages described so far in S. cerevisiae. The activation of the TORC1 pathway by a proline-to-glutamine upshift was indirectly evaluated using our system and the traditional direct methods based on immunoblot (Sch9 and Rps6 phosphorylation). Regardless of the different molecular readouts obtained with both methodologies, the general results showed a wide phenotypic variation between the representative strains analysed. Altogether, this easy-to-use assay opens the possibility to study the molecular basis for the differential TORC1 pathway activation, allowing to interrogate a larger number of strains in the context of nitrogen metabolism phenotypic differences.</AbstractText><CopyrightInformation>© 2018 John Wiley & Sons, Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kessi-Pérez</LastName><ForeName>Eduardo I</ForeName><Initials>EI</Initials><Identifier Source="ORCID">0000-0003-4225-9486</Identifier><AffiliationInfo><Affiliation>Departamento de Ciencia y Tecnología de los Alimentos, Universidad de Santiago de Chile (USACH), Santiago, Chile.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Salinas</LastName><ForeName>Francisco</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Centro de Estudios en Ciencia y Tecnología de Alimentos (CECTA), Universidad de Santiago de Chile (USACH), Santiago, Chile.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Millennium Institute for Integrative Biology (iBio), Santiago, Chile.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Molinet</LastName><ForeName>Jennifer</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Departamento de Ciencia y Tecnología de los Alimentos, Universidad de Santiago de Chile (USACH), Santiago, Chile.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>González</LastName><ForeName>Asier</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Biozentrum, University of Basel, Basel, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Muñiz</LastName><ForeName>Sara</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Departamento de Biotecnología de los Alimentos, Instituto de Agroquímica y Tecnología de los Alimentos (CSIC), Paterna, Valencia, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guillamón</LastName><ForeName>José M</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Departamento de Biotecnología de los Alimentos, Instituto de Agroquímica y Tecnología de los Alimentos (CSIC), Paterna, Valencia, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hall</LastName><ForeName>Michael N</ForeName><Initials>MN</Initials><AffiliationInfo><Affiliation>Biozentrum, University of Basel, Basel, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Larrondo</LastName><ForeName>Luis F</ForeName><Initials>LF</Initials><AffiliationInfo><Affiliation>Millennium Institute for Integrative Biology (iBio), Santiago, Chile.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martínez</LastName><ForeName>Claudio</ForeName><Initials>C</Initials><Identifier Source="ORCID">0000-0001-8564-9287</Identifier><AffiliationInfo><Affiliation>Departamento de Ciencia y Tecnología de los Alimentos, Universidad de Santiago de Chile (USACH), Santiago, Chile.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centro de Estudios en Ciencia y Tecnología de Alimentos (CECTA), Universidad de Santiago de Chile (USACH), Santiago, Chile.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>0946</GrantID><Agency>CSIC/i-LINK+</Agency><Country>International</Country></Grant><Grant><Agency>Instituto Milenio iBio - Iniciativa Científica Milenio-MINECON</Agency><Country>International</Country></Grant><Grant><GrantID>21150700</GrantID><Agency>CONICYT/Beca Doctorado Nacional</Agency><Country>International</Country></Grant><Grant><GrantID>REDI170239</GrantID><Agency>CONICYT/PCI</Agency><Country>International</Country></Grant><Grant><GrantID>11170158</GrantID><Agency>CONICYT/FONDECYT</Agency><Country>International</Country></Grant><Grant><GrantID>1150522</GrantID><Agency>CONICYT/FONDECYT</Agency><Country>International</Country></Grant><Grant><GrantID>EQM130158</GrantID><Agency>CONICYT/FONDEQUIP</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>09</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Yeast</MedlineTA><NlmUniqueID>8607637</NlmUniqueID><ISSNLinking>0749-503X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C489218">RPL26A protein, S cerevisiae</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012269">Ribosomal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029701">Saccharomyces cerevisiae Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.13.12.-</RegistryNumber><NameOfSubstance UI="D008156">Luciferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.11.1</RegistryNumber><NameOfSubstance UI="D000076222">Mechanistic Target of Rapamycin Complex 1</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="N">Fermentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="Y">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008156" MajorTopicYN="N">Luciferases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000076222" MajorTopicYN="N">Mechanistic Target of Rapamycin Complex 1</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010766" MajorTopicYN="N">Phosphorylation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012269" MajorTopicYN="N">Ribosomal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029701" MajorTopicYN="N">Saccharomyces cerevisiae Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="Y">Signal Transduction</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Saccharomyces cerevisiae</Keyword><Keyword MajorTopicYN="Y">TORC1 pathway</Keyword><Keyword MajorTopicYN="Y">microculture</Keyword><Keyword MajorTopicYN="Y">natural variation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>02</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>07</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>07</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>8</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>4</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>8</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30094872</ArticleId><ArticleId IdType="doi">10.1002/yea.3351</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Chili</li><li>Espagne</li><li>Suisse</li></country></list><tree><country name="Chili"><noRegion><name sortKey="Kessi Perez, Eduardo I" sort="Kessi Perez, Eduardo I" uniqKey="Kessi Perez E" first="Eduardo I" last="Kessi-Pérez">Eduardo I. Kessi-Pérez</name></noRegion><name sortKey="Larrondo, Luis F" sort="Larrondo, Luis F" uniqKey="Larrondo L" first="Luis F" last="Larrondo">Luis F. Larrondo</name><name sortKey="Larrondo, Luis F" sort="Larrondo, Luis F" uniqKey="Larrondo L" first="Luis F" last="Larrondo">Luis F. Larrondo</name><name sortKey="Martinez, Claudio" sort="Martinez, Claudio" uniqKey="Martinez C" first="Claudio" last="Martínez">Claudio Martínez</name><name sortKey="Martinez, Claudio" sort="Martinez, Claudio" uniqKey="Martinez C" first="Claudio" last="Martínez">Claudio Martínez</name><name sortKey="Molinet, Jennifer" sort="Molinet, Jennifer" uniqKey="Molinet J" first="Jennifer" last="Molinet">Jennifer Molinet</name><name sortKey="Salinas, Francisco" sort="Salinas, Francisco" uniqKey="Salinas F" first="Francisco" last="Salinas">Francisco Salinas</name><name sortKey="Salinas, Francisco" sort="Salinas, Francisco" uniqKey="Salinas F" first="Francisco" last="Salinas">Francisco Salinas</name></country><country name="Suisse"><noRegion><name sortKey="Gonzalez, Asier" sort="Gonzalez, Asier" uniqKey="Gonzalez A" first="Asier" last="González">Asier González</name></noRegion><name sortKey="Hall, Michael N" sort="Hall, Michael N" uniqKey="Hall M" first="Michael N" last="Hall">Michael N. Hall</name></country><country name="Espagne"><noRegion><name sortKey="Mu Iz, Sara" sort="Mu Iz, Sara" uniqKey="Mu Iz S" first="Sara" last="Mu Iz">Sara Mu Iz</name></noRegion><name sortKey="Guillam N, Jose M" sort="Guillam N, Jose M" uniqKey="Guillam N J" first="José M" last="Guillam N">José M. Guillam N</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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{{Explor lien
|wiki= Bois
|area= RapamycinFungusV1
|flux= Main
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|clé= pubmed:30094872
|texte= Indirect monitoring of TORC1 signalling pathway reveals molecular diversity among different yeast strains.
}}
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